chaps detergent cmc

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chaps detergent cmc

Detergent的性质及其在膜蛋白中的应用 - 知乎- chaps detergent cmc ,查看水通道相关蛋白和抗体近年来,膜蛋白研究取得了明显的进展,这离不开研究膜蛋白的相关工具和试剂的进步[1]。其中,去垢剂在膜蛋白的提取、纯化和操作中起了重要作用。它们的两亲性质(amphiphilic nature)使…CHAPS | DojindoProduct Description of CHAPS and CHAPSO. CHAPS and CHAPSO are zwitter ionic detergents and have cholic acid and sulfobetaine moieties in their structures. Their low background absorbance in the UV region is an attractive feature for the UV monitoring of membrane proteins. The CMC values of both CHAPS and CHAPSO are 8 mM.



CHAPS detergent - WikiMili, The Best Wikipedia Reader

CHAPS is a zwitterionic surfactant used in the laboratory to solubilize biological macromolecules such as proteins.It may be synthesized from cholic acid [1] and is zwitterionic due to its quaternary ammonium and sulfonate groups; it is structurally similar to certain bile acids, such as taurodeoxycholic acid and taurochenodeoxycholic acid.It is used as a non-denaturing …

18 Fast Facts about CHAPS Detergent - AG Scientific

CHAPS is a high CMC detergent that can be easily removed by dialysis while those with low CMCs are typically removed by adsorption to hydrophobic beads. References : Comparative study of the interaction of CHAPS and Triton X-100 with the erythrocyte membrane P.M. Rodia, M.D. Bocco Gianello a , M. Corregido a , A.M. Gennaro

CHAPS | Zwitterionic Detergents | Detergents & …

CHAPS, zwitterionic detergent Type: Zwitterionic detergent Full Name: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate Mol. Formula: C32H58N2O7S Mol Weight: 614.9 Form: White solid Purity: >99% Solubility: …

CHAPS Detergent | CAS 75621-03-3 - Hopax Fine …

CHAPS is a non-denaturing detergent for the solubilization of membrane proteins and breaking protein-protein interactions. The structure of this surfactant combines the useful properties of both the sulfobetaine-type and the bile salt …

CHAPS detergent - Wikipedia

CHAPS is an abbreviation for 3- [ (3- ch olamidopropyl)dimethyl a mmonio]-1- p ropane s ulfonate. A related detergent, called CHAPSO, has the same basic chemical structure with an additional hydroxyl functional group; its full chemical name is 3- [ (3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate.

CHAPS 去污剂 (3-((3-胆酰胺丙基) 二甲基氨基丙基)-1- …

Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteinsHAPS is sulfobetaine derivative of cholic acid. This zwitterionic detergent is useful for membrane protein solub …

CHAPS - Dojindo Molecular Technologies, Inc.

Product Description of CHAPS and CHAPSO. CHAPS and CHAPSO are zwitter ionic detergents and have cholic acid and sulfobetaine moieties in their structures. Their low background absorbance in the UV region is an attractive feature for the UV monitoring of membrane proteins. The CMC values of both CHAPS and CHAPSO are 8 mM.

Anatrace Cholesterol Derivatives

Molecular Formula: C 32 H 58 N 2 O 7 S. CAS Number: [75621-03-3] Formula Weight: 614.9. CMC: (H 2 O) ~ 8 mM (1) (0.49%) Aggregation Number: (H 2 O) ~ 10 (2) dn/dc: (H 2 O) 0.1323 ml/gm (3) Purity: ≥ 99% (by HPLC analysis) pH: 5-8 (1% solution in water) Solubility: ≥ …

DETERGENTS AND THEIR USES IN MEMBRANE PROTEIN …

(CMC); the concentration of detergent above which monomers self-assemble into non-covalent aggregates (called micelles) [1, 30, 31]. The CMC actually does not occur at a single concentration, but rather, over a narrow concentration range. When the total detergent concentration is below the CMC, detergent monomers are free in bulk solution.

Dependence of critical micelle concentration of a ...

Aug 05, 1996·Although the mechanism for such salt-dependent CMC for CHAPS needs to be worked out, it could be due to the salting out of the non-polar moiety of CHAPS, as has been shown for other uncharged detergents [111. The steady decrease in CMC of CHAPS with increasing salt concentration is shown in Table 1.

CHAPS | Avanti Polar Lipids

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS’ small micellar molecular weight (6,150; aggregation number = 10) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis.

CHAPSO Detergent | CAS 82473-24-3 - Hopax Fine Chemicals

Hopax is a manufacturer and supplier of CHAPSO (CAS 82473-24-3), a zwitterionic detergent widely used for protein preparation, solubilization and separation. Properties of Chapso detergent: Chapso is a zwitterionic detergent similar to Chaps , however, Chapso is more soluble and particularly better for the solubilization of integral membrane ...

18 Fast Facts about CHAPS Detergent - AG Scientific

CHAPS is a high CMC detergent that can be easily removed by dialysis while those with low CMCs are typically removed by adsorption to hydrophobic beads. References : Comparative study of the interaction of CHAPS and Triton X-100 with the erythrocyte membrane P.M. Rodia, M.D. Bocco Gianello a , M. Corregido a , A.M. Gennaro

Detergents - Dojindo EU GmbH

Product Description of CHAPS and CHAPSO CHAPS and CHAPSO are zwitter ionic detergents and have cholic acid and sulfobetaine moieties in their structures. Their low background absorbance in the UV region is an attractive feature for the UV monitoring of membrane proteins. The CMC values of both CHAPS and CHAPSO are 8 mM.

Detergent Properties Detergent Analysis CMC - Anatrace - 豆丁网

Aug 18, 2016·Detergent Properties Detergent Analysis CMC - Anatrace. ... (0.49%) ~10 C316S 76,112 CHAPS,Sol-Grade 614.9~8(0.49%) ~10 C317 76,113 CHAPSO,Anagrade 630.9~8(0.50%) ~11 C408 46 C-HEGA-8,Anagrade 349.5~277(9.7%) C40946 C-HEGA-9,Anagrade 363.5~108(3.9%) C41046 C-HEGA-10,Anagrade 377.5~35(1.3%) C41147 C …

BIG CHAPS | CAS 86302-22-2 | Detergent | P212121 …

CAS Number: 86302-22-2. Chemical Formula: C 42 H 75 N 3 O 16. Molecular Weight: 878.1 g/mol. Big CHAPS is a zwitterionic detergent often used to solublize proteins. Critical miceller concentration (CMC) water ~2.9 mM. Free …

What are the easiest detergents to remove after …

Jan 01, 2009·High CMC detergent (like TX100, OG, CHAPS) are easier to remove than low CMC detergent (like DDM, C12E8) as it could be easy to dilute a bit your sample to decrease their concentrations below ...

CHAPS ≥98% | Sigma-Aldrich

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis.

Detergents Product Guide - carbosynth

CMC of non-ionic detergent is usually lower than for ionic detergents, and CMC of zwitterionic detergents is lower than CMC of ionic detergents. When working with membrane proteins the detergent concentration must be at least double the CMC. The average number of detergent molecules present in a micelle is defined as micelle aggregation number.

去垢剂的选择 – 聚生物

生物医学实验室使用的去垢剂是温和的表面活性剂(表面作用剂),用于细胞溶解(即细胞膜破裂)和释放细胞内材料。. 它们是两亲性分子,同时含有亲水和疏水区域。. 这种两亲性使得去垢剂能够破坏蛋白质、蛋白质脂质和脂质的结合、变性蛋白质和其他大 ...

Dependence of critical micelle concentration of a ...

Aug 05, 1996·Although the mechanism for such salt-dependent CMC for CHAPS needs to be worked out, it could be due to the salting out of the non-polar moiety of CHAPS, as has been shown for other uncharged detergents [111. The steady decrease in CMC of CHAPS with increasing salt concentration is shown in Table 1.

膜タンパク質可溶化剤 CHAPS 同仁化学研究所

性質. 両性界面活性剤:cmc=8 mmol/L. 膜タンパク質の溶解剤としての好ましい条件の一つに非イオン性であることがあげられ、例えばTriton X-100などが用いられているが、溶解効率、除去の難しさ等の点で必ずしも満足なものとは言えない。. ここに紹介するCHAPS ...

CHAPS Detergent | CAS 75621-03-3 - Hopax Fine …

CHAPS is a non-denaturing detergent for the solubilization of membrane proteins and breaking protein-protein interactions. The structure of this surfactant combines the useful properties of both the sulfobetaine-type and the bile salt …

Safe concentrations of CHAPS for performing size-exclusion ...

Jan 01, 1999·You can start trying with 2-3 fold the CMC of CHAPS (8 mM, so keeping 1% (16 mM) could be a good start). CHAPS micelles are pretty small though, so even having some excess must be good for you.